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| Cunha,L.K.H.; Kashima,S.; Amarante,M.F.C.; Haddad,R.; Rodrigues,E.S.; Silva,K.L.T.; Lima,T.A.; Castro,D.B.; Brito,F.C.; Almeida,E.G.; Covas,D.T.; Malheiro,A.. |
| Few studies have reported the molecular epidemiological characterization of HIV-1 in the Northern region of Brazil. The present study reports the molecular and epidemiological characterization of 31 HIV-1 isolates from blood donors from the State of Amazonas who donated blood between April 2006 and March 2007. Serum/plasma samples from all donors were screened for HIV antibodies by ELISA and the results confirmed by Western blot analysis. Genomic DNA was extracted from the buffy coat using the Super Quik-Gene-DNA Isolation kit. Nested PCR was performed on the env, gag, and pol regions of HIV-1 using the Gene Amp PCR System 9700. Sequencing reactions were performed using the inner PCR primers and the DYEnamic™ ET Dye Terminator Kit, and phylogenetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HIV-1; Subtypes; Phylogenetic analysis; Blood donors; Molecular and epidemiological characterization. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200003 |
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| Pinto,M.T.; Nicolete,L.D.F.; Rodrigues,E.S.; Palma,P.V.B.; Orellana,M.D.; Kashima,S.; Covas,D.T.. |
| Multipotent mesenchymal stromal cells (MSCs) were first isolated from bone marrow and then from various adult tissues including placenta, cord blood, deciduous teeth, and amniotic fluid. MSCs are defined or characterized by their ability to adhere to plastic, to express specific surface antigens, and to differentiate into osteogenic, chondrogenic, adipogenic, and myogenic lineages. Although the molecular mechanisms that control MSC proliferation and differentiation are not well understood, the involvement of microRNAs has been reported. In the present study, we investigated the role of miR-125b during osteoblastic differentiation in humans. We found that miR-125b increased during osteoblastic differentiation, as well as Runx2 and ALPL genes. To study... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Multipotent mesenchymal stromal cells; Osteoblastic differentiation; Hsa-miR-125b. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800676 |
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